3D culture systems, like spheroids and organoids, represent useful in vitro models for drug screening and, more broadly, for disease modelling. The possibility to characterize spheroid’s biophysics would considerably increase the knowledge of critical factors in 3D biology, such as cellular density and size-dependent mass transport. In this regard, the dataset of biophysical observables, collected with the W8 Physical Cytometer, paves the way to a new, label-free method for the analysis of 3D cell culture-based assays.

The format is a roundtable discussion comprising current users and experts utilising CellDynamics novel patented W8 system.

Marianna Kruithof-de Julio PhD Director of Organoid Core @ University of Bern (Bern, CH)

Spartaco Santi PhD Head of Digital Microscopy Center @ Institute of Molecular Genetics – CNR (Bologna, IT)