Description
The RIBOPROTECT Hu RNase Inhibitor Lyo-Ready is a 50 kDa recombinant human placental protein expressed in Escherichia coli, in a special formulation (without glycerol). It inhibits ribonuclease (RNase) activity of common eukaryotic enzymes such as RNase A, RNase B, RNase C by non-covalent binding in a 1:1 ratio. RIBOPROTECT Hu is intended for use in applications where the presence of RNases may cause a hazard to RNA quality and experiment results, e.g. in RNA isolation, cDNA synthesis, RT-PCR, RT-qPCR, RT-LAMP, in vitro transcription and translation, or RNase-free monoclonal antibody preparation. RIBOPROTECT Hu shows no activity towards RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H. It is compatible with DNA Polymerases and AMV or M-MuLV Reverse Transcriptases. Formulation of RIBOPROTECT Hu RNase Inhibitor Lyo-Ready enables its usage directly in the lyophilization process.
Features and advantages
- Formulation without glycerol for efficient freeze-drying
- High functionality after lyophilization and reconstitution
- Stable at 37°C for at least 4 weeks with no loss of activity
- Fully stable for at least 6 hours in 50°C
- Completely inhibits RNase A, RNase B, and RNaseC activity
- Possibility of single-reaction or in-bulk lyophilization process
- No influence on the polymerase or reverse transcriptase activity
- Free of DNase and RNase activity
- Active in diverse reaction conditions and in various buffers
- Active over a broad range of pH and DTT
- High concentration formulation available (100 kU/ml)
Applications
- cDNA synthesis, RT-PCR, RT-qPCR, RT-LAMP
- Optimal RNA protection in lyophilized diagnostic kits
- RNA isolation and purification
- in vitro transcription and translation
- RNase-free monoclonal antibody preparation
Unit definition
One unit is defined as the amount of enzyme required to inhibit the activity of 5 ng RNase A by 50%.