LunaGel – Photocrosslinkable Extracellular Matrix – Fish Gelatin

LunaGel – Photocrosslinkable Extracellular Matrix – Fish Gelatin

This LunaGel™ Photocrosslinkable Extracellular Matrix (ECM) product is based on chemically modified cold water fish skin gelatin. Compared to mammalian gelatin-based matrices, LunaGel™ Fish Gelatin has a lower viscosity and is liquid at room temperature, making it extremely easy to use without heating or cooling. Fully compatible with automated liquid handling.

The major components of LunaGel™ include the ECM proteins collagen type I, III, IV, and V, as well as connective tissue glycoproteins and proteoglycans. LunaGel™ retains the intrinsic cell-instructive bioactivity of natural ECMs facilitating cell attachment, proliferation, differentiation, migration, and proteolytic degradation. LunaGel™’s unique photocrosslinking technology allows unprecedented control over matrix porosity and stiffness, allowing researchers to replicate the physicochemical properties of a variety of healthy and diseased tissues in 3D cell culture applications.

 

LunaGel™ is supplied as a sterile solution with freeze-dried photoinitiator. Available as Low Stiffness (0 – 6.5 kPa) and High Stiffness kit (0 – 25 kPa).

Reconstitute the photoinitiator in PBS, mix with LunaGel™ ECM and cells, and photocrosslink the solution using the  Luna Crosslinker™ to form 3D cell culture models. By controlling the duration of light exposure you can produce hydrogels with a specified stiffness. Add your favourite culture media and you’re culturing in 3D!

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Description

LunaGel™ ECM has been successfully used in a wide range of applications including cell attachment and proliferation, stem cell culture and differentiation, mechanotransduction assays, cancer spheroid assays, angiogenesis assays, 3D bioprinting, tissue engineering, and more.

How It Works

LunaGel - How It Works