|Name||Part Number||Pack Size|
|LongNova-RED DNA Polymerase||RP281R||200 U|
LongNova-RED DNA Polymerase is a mixture of thermostable Pwo and Taq polymerases with the addition of an inert red dye which facilitates accurate low volume pipetting and is an indicator of enzyme addition. The dye has no adverse effect on the outcome of PCR; yields are the same as with the standard LongNova DNA Polymerase.
The thermostable LongNova DNA Polymerase catalyses DNA synthesis in a 5’-3’ direction and shows a 3’-5’ exonuclease activity. It is characterized by high processivity and proofreading properties. It is ideal for long range PCR (up to 20 kb) and GC-rich template amplification applications.
Use of the LongNova-RED DNA Polymerase decreases the risk of error during a reaction set up, e.g. skipping the polymerase or inaccurate reagent mixing. In addition, the PCR product can be applied directly to a gel after amplification without the need to mix it with a loading buffer. This reduces the time required to perform agarose or polyacrylamide electrophoresis.
Features and advantages
– Wide range of product sizes – from 2 to 20 kb
– High proofreading properties (3′-5’ exonuclease activity)
– Facilitate PCR reaction set-up
– Direct gel loading
– Long PCR products
– Molecular cloning
– Site-directed mutagenesis and other methods which require high fidelity