Phone

01904 900786

Address

The Catalyst, Baird Lane, York, YO10 5GA

TaqNova Polymerase

Universal and easy-to-use DNA polymerase which works rapidly and effectively in various PCR conditions

Description

Name Part Number Pack Size
TaqNova Polymerase RP702 200 U (2 U/µl)
  RP702A 200 U (5 U/µl)
  RP705 500 U (2 U/µl)
  RP705A 500 U (5 U/µl)
  RP710 1000 U (2 U/µl)
  RP710A 1000 U (5 U/µl)
  RP725 2500 U (2 U/µl)
  RP725A 2500 U (5 U/µl)

TaqNova DNA Polymerase is a 94 kDa recombinant thermostable Taq DNA polymerase isolated from Thermus aquaticus. It is recommended for a wide range of applications, which require DNA synthesis in extremely high temperatures. The TaqNova polymerase is a universal and easy-to-use DNA polymerase, which works rapidly and effectively in various PCR conditions. The enzyme catalyses DNA synthesis in a 5’-3’ directions, it does not show a 3’-5’ exonuclease activity, however it has a 5’-3’ exonuclease activity.

Features:

– recombinant enzyme of high purity

– extreme yield with minimal amounts of enzyme and little optimization

– catalyses DNA polymerization at 72°C in the presence of Mg2+ ions

– half-life of the enzyme is 45 minutes at 95°C

– shows 5’-3’ exonuclease activity

– does not have 3’-5’ exonuclease activity

– adds A on the 3’ ends

Applications:

– efficient amplification of short and medium size sequences

– diagnostic PCR

Specification

Concentration:

2 U/µl or 5 U/µl

Contents:

TaqNova polymerase; 10x TaqNova reaction buffer with KCl; 10x TaqNova reaction buffer with (NH4)2SO4; 50 mM MgCl2

10x TaqNova reaction buffer with KCl (without Mg2+):

100 mM Tris-HCl (pH 8.8, 25°C), 500 mM KCl, 0.8% (v/v) Nonidet P40

10x TaqNova reaction buffer with (NH4)2SO4 (without Mg2+):

750 mM Tris-HCl (pH 8.8 w 25°C), 200 mM (NH4)2SO4, 0,1% (v/v) Tween 20

Storage Buffer:

20 mM Tris-HCl (pH 8.0 at 25°C), 200 mM KCl, 0.1 mM EDTA, 2 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.

Storage conditions:

Storage at -20ºC

Shipping conditions:

Shipping on dry or blue ice

Quality control:

Nuclease-free. Extensively tested for PCR applications.

Literature

TaqNova Booklet