VERLO Image Guided Cell Sorter

VERLO Image Guided Cell Sorter

The VERLO™ Image-Guided Cell Sorter is providing conventional flow cytometry and cell sorting along with the ability to perform imaging analysis to identify and sort cells based on morphology, subcellular localization and more.

The VERLO™ instrument expands the capabilities of the WOLF G2 gentle benchtop microfluidic cell sorting. With two lasers and nine colors, plus 3 label-free parameters, it maintains simple workflows for either bulk sorting or single-cell dispensing into 96- or 384-well plates with an integrated cell dispensing and sample cooling.

 

Description

The VERLO™ Image-Guided Cell Sorter is providing conventional flow cytometry and cell sorting along with the ability to perform imaging analysis to identify and sort cells based on morphology, subcellular localization and more.

The VERLO™ instrument expands the capabilities of the WOLF gentle benchtop microfluidic cell sorting. With two lasers and nine colors, plus 3 label-free parameters, it maintains simple workflows for either bulk sorting or single-cell dispensing into 96- or 384-well plates with an integrated cell dispensing and sample cooling.

 
 

Adding Image Analysis to Gentle Microfluidic Cell Sorting

Spatial Information – Captures images of each cell. In addition to fluorescence intensity, the VERLO provides spatial information such as cell morphology and marker localization

 

Enhanced Resolution – Imaging of subcellular structures and analysis of spatial variations in brightfield, darkfield, and fluorescent images

 

Increased Precision Sorting – Following the acquisition and processing of cell images, imaging features are incorporated into the gating strategy to define cell phenotypes

 

Multivariate Datasets – Combines high-throughput sampling with single-cell image acquisition and measures large number of features from cell images, providing more comprehensive data

 
stem cell key applications
 

VERLO™ Image Sorting Technology 12-Parameter Image-Guided Sorting

With 488 nm and 561 nm lasers to detect up to 9 fluorescent markers and 3 channels of label-free information, the VERLO Image-Guided Cell Sorter can identify key structures, such as golgi and nuclei, internalization, overall morphology, and nuanced texture features that can discriminate live-dead—as well as the physiology of the cells.

VERLO Features 

Once you have decided on which morphological features of the cells you want to sort, you can gate on image features such as Area vs Max Intensity, just like you would when sorting using FSC vs BSC. The image features are calculated in real-time, enabling you to begin sorting your samples without having to first analyze the whole sample and then run it through the instrument again.

 
VERLO Data

Microfluidics

The VERLO™ Image-Guided Cell Sorter uses patented, microfluidic-based sorting with robust laser-excitation and sensitive PMT detectors to isolate mammalian cells, microbes, plant cells and more. A gentle and precise piezoelectric actuator directs cells into collection channels and allows analysis and sorting in a disposable format. This eliminates sample-to-sample contamination and biohazard exposure or cleanup. This technology allows the VERLO™ to sort up to 200 cells per second with high accuracy and effective recovery. Deposit 1 to 100 cells per well in a 96- or 384 well plate using a single-cell sorting cartridge and integrated Single-Cell Dispenser. CellVantage Software VERLO™ integrated platform uses patented, microfluidic-based sorting with robust laser-excitation and sensitivePMT detectors to isolate mammalian cells, microbes, plant cells and more. Agentle and precise piezoelectric actuator directs cells into collection channels and allows analysis and sorting in a disposable format. This eliminates sample-to-sample contamination and biohazard exposure or cleanup.
  • Unique to NanoCellect are our disposable cartridges that allow for bulk sorting or single-cell sorting. 
  • The sorting cartridges use a piezoacoustic actuator that gently directs cells into collection channels; an embedded cell sorting verification system gives instant feedback of sorting accuracy. 
  • This technology allows the VERLO™ to sort up to 200 cells per second with high accuracy and effective recovery. 
  • Sort two selected cell populations with bulk sorting while the remainder of cells collects in a third channel. 
  • Deposit 1 to 100 cells per well in a 96- or 384 well plate using a single-cell sorting cartridge and integrated Single-Cell Dispenser.Microfluidic Cartridge

VERLO™ Early Access Program

Are you ready to advance the future of image-guided cell sorting?

NanoCellect is looking for scientific thought leaders and technology enthusiasts in the Stem Cell Research & Development and Flow Cytometry industries who want early, exclusive access to an innovative product at the forefront of image-guided cell sorting technology, before it is made available to the general market.

The VERLO Early Access Program provides early access for the yet-to-be-released VERLO image-guided cell sorting technology platform through documented purchase intent.

Program Benefits

  • Early Access at a Discounted Price
  • Influence Product Development
  • Travel Awards
  • Scientific Promotion

Specification

VERLO-Image-Guided-Cell-Sorter-Brochure-April-2024

Applications

VERLO-Image-Guided-Cell-Sorter-Brochure-April-2024

NanoCellect WOLF G2 Cell Sorter Brochure (Jan 2023)

Literature

VERLO-Image-Guided-Cell-Sorter-Brochure-April-2024

NanoCellect WOLF G2 Cell Sorter Brochure (Jan 2023)