On-chip Droplet Generator allows mass production of micrometre-sized water-in-oil (W/O) emulsion droplets and gel microdrops (GMDs) with a unique microfluidic chip. The individual droplets function as miniaturised vessels for reactions such as microbial/cell cultivation and genetic analysis. Droplet technology is now recognised as a prominent high-throughput screening method allowing single cell analysis or simultaneous assay of more than 1 million samples.
On-chip Droplet Generator allows production of stable and monodisperse droplets.
Structure of microchannel
Easy adjustment of droplet size
Droplet size can be easily adjusted between 60 µm and 220 µm in diameter by regulating the pressure applied to the sample and oil reservoirs.
Real-time monitoring of droplet generation
Droplet size can be viewed and monitored real-time with the supplied monitoring device.
Types of droplets generated
On-chip Droplet Generator can produce two types of droplets: water-in-oil (W/O) emulsion droplets and gel microdrops (GMDs).
W/O droplet
GMD
No. of droplets
≥ 1 million droplets
≥ 1 million droplets
Contents
Aqueous liquid (medium, etc.)
Solid (agarose, gelatin, etc.)
Transfer of substances
Maintained inside (depending on polarity)
Extracellular fluid including reagent infiltrates the inside
Usage
Environmental microbial screening, etc.
Variant screening, etc.
Immediately after encapsulation
After cultivation
E. coli
Lung cancer-derived cell line
Utilisation of On-chip Droplet Generator and On-chip Sort
On-chip Sort utilises microfluidic chip technology that allows oil to be used as sheath fluid, which makes the selection of W/O droplets with microorganisms possible.
Specification
Main Unit Size
Size
270 (W) x 270 (D) x 280 (H) mm
Weight
10kg
Main Unit
Liquid delivery pressure
0.1 – 80 kPa
Maximum liquid delivery line
3 lines (sample, oil, other reagents)
Droplet size generated
Diameter 60 – 220 μm (can be adjusted discretionally)
Generation rate
Approx. 100,000 droplets/3 min (for generation of 100 μm droplets)
Regulation
Laptop PC (Windows 10)
Power input
AC 100-240V, 50/60Hz
Power consumption
1.2A typ (ACIN 100V)
Monitoring Unit
Size
215 (W) x 310 (D) x 430 (H) mm
Accessories
Monitor camera, Peltier unit, Controller
Temperature range
ー5 ～ 70℃
Ambient environment
Temperature 5 – 40˚C, humidity ≤ 85% RH
Power input
DC24 V
Power consumption
6.5 A max
Applications
“Droplets” can be easily generated in large quantities and have the ability to encapsulate microorganisms or cells individually, allowing for the analysis of a wide variety of samples simultaneously and in parallel. Therefore, Droplet has been used in various fields, including high-throughput screening (HTS), making use of various advantages such as accelerated generation and analysis, small-bioreactor culture, and single-cell analysis.
Advantages of Droplet Technology
Each droplet (picoliter-scale) functions as each well (microliter-scale) of a well-plate.
Over one million droplets (reaction compartments) can be stored in a single tube.
Significant savings can be achieved in analysis time, reagent consumption, and incubation space.
Target droplet can be screened by On-chip Sort/On-chip Droplet Selector
Micro-titer plate (Conventional method)
Droplet (Next generation method)
One reaction volume
100μL/1well
100pL ～ 1 drop
For analysis of one million samples
2,600 plates (384-well)
One tube only
Analysis Speed
Long (several days)
Short (several hours)
Cost
High
Low
Applications
Water-in-Oil (W/O) Droplets
Schematic of a W/O droplet (left) and microscopic image of environmental microorganism culture (right)
Structure: Emulsion in which water droplets (e.g. the medium) are dispersed in oil and stabilized by surfactants.
Features: Microorganisms/cells grow and metabolites accumulate in each droplet as they are compartmentalized by oil. The addition of substrates allows for the detection of metabolites along with the cultivation.
Use: Microbial culture, screening of environmental microbes/mutant strains, directed evolution of enzymes, etc.
Single-cell encapsulation and cultivation allow massively parallel screening based on the growth, protein expression, and secretion amount.
Luu, X. C., Shida, Y., Suzuki, Y., Sato, N., Nakamura, A., Ogasawara, W.. New Biotechnology 2022 72, 149-158.
Novel Microorganisms Screening
Microorganisms from industrial wastewater with specific protease activity
As a tool for isolating novel microorganisms from diverse environments, including soil, hydrosphere, and extreme environments.
Nakamura, A., Honma, N., Tanaka, Y., Suzuki, Y., Shida, Y., Tsuda, Y., Hidaka, K., Ogasawara, W., Analytical Chemistry 2022 94 (5), 2416-2424.
Cell-Cell Interaction
Co-encapsulation of GFP-expressing pathogens and soil microorganisms. Green fluorescence is attenuated in droplets showing antagonistic effects.
Droplet Library can be easily prepared containing combinations of various types of bacteria by taking advantage of the feature of high-throughput droplet generation.
The results are obtained in conjunction with Dr. Yasunori Ichihashi and Dr. Megumi Narukawa, RIKEN BioResource Research Center
Gel Microdrops (GMD)
Schematic of a GMD (left) and microscopic image of cancer cell culture (right)
Structure: Droplets with gelled interior.
Features: Substances are highly permeable both inside and outside the droplet structure because the droplets can be dispersed in an outer aqueous phase, such as a culture medium, rather than in oil, while relatively large materials such as cells, beads, and genomes are retained by the gel as a scaffold.
Use: Cell culture, screening of mutant strains/antibody-producing cells, microbial interaction studies, etc.
Isolation of Protein High-Producing Strains
Recombinant protein-producing budding yeast
High-throughput screening of high-producing strains from a large number of candidates such as UV mutant libraries
Fujitani, H., Tsuda, S., Ishii, T., Machida, M., bioRxiv 2019
Screening Based on Substance Production
Oleaginous microorganisms stained with BODIPY
Staining of microorganisms after GMD cultivation allows screening based on substance production such as lipids.
Tanaka, Y., Nakamura, A., Suzuki, Y., Maruta, K., Shida, Y., Ogasawara, W. , Journal of Biotechnology 2022 358, 46-54.
Elucidation of the Gut Microbiota Function
Elucidation of the gut microbiota function
High-throughput screening to elucidate the function of diverse gut microbiota
The results are obtained in conjunction with Prof. Shinji Fukuda, Institute for Advanced Biosciences, Keio University
Droplet Sorting by Microfluidics Chip
On-chip Sort / On-chip Droplet Selector enables the screening of droplets of interest based on enzyme reaction, proliferative activity, antibody reaction, etc.
Detection and Screening of Droplets Encapsulating Microorganism
A major challenge in obtaining pure cultures of environmental microorganisms has been detecting and isolating droplets containing microorganisms with various growth rates from millions of droplets. To solve this problem, Dr. Noda and his team at the National Institute of Advanced Industrial Science and Technology (AIST) developed FNAP-sort (fluorescent nucleic acid probe in droplets for bacterial sorting), a method that utilizes fluorescence to detect droplets containing grown microorganisms. In this method, a fluorescent nucleic acid probe, which is quenched by FRET (fluorescence resonance energy transfer), is cleaved by RNase generated during microbial growth, resulting in the elimination of FRET and an increase in fluorescence emission.
FNAP-sort workflow and proof of principle
(A) FNAP-sort workflow, (B) microscopic images of droplets immediately after generation, (C) microscopic images of droplets after one day of incubation, (D) fluorescence histogram of droplets after one day of incubation, and (E) microscopic images of sorted droplets. Upper panels: dark-field observation; lower panels: fluorescence observation for (B), (C), and (E).
Ota, Y., Saito, K., Takagi, T., Matsukura, S., Morita, M., Tsuneda, S., Noda, N., PLOS One 2019 14(4), e0214533.
Saito, K., Ota, Y., Tourlousse, D. M., Matsukura, S., Fujitani, H., Morita, M., Tsuneda, S., Noda, N., Scientific Report 2021 11(1), 9506.
Sample List
Sorting of w/o droplets or GMD encapsulating cells
With conventional cell sorters, handling water-in-oil droplets is not possible due to the difficulty in using oil as the sheath fluid and the droplets’ lack of resistance to physical shock.…
FEATURES Microfluidic chip technology On-chip Sort integrates the entire process from sample analysis to separation on a small microfluidic chip with measurements of 5.5 cm × 4.0 cm. In contrast to…
