Description

5Х Fast Probe qPCR Mastermix is a universal ready-to-use reagent for quantitative and/or qualitive PCR with real-time detection of the results. The qPCR Mix contains optimized components and inactivated DNA polymerase supplied in a proprietary reaction buffer. 5Х Fast Probe qPCR Mastermix is optimized for DNA/LNA hydrolysis probes based on the 5 ́-3 ́ exonuclease activity. DNA Polymerase is activated by 2 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. 5X Fast Probe qPCR Mastermix consists of the following components:
– thermostable DNA polymerase with a hot-start;
– dNTP;
– optionally ROX reference dye (final concentration is 0.5 μM (High ROX) or 0.03 μM (Low ROX) depending of qPCR instrument);
– MgCl2 (final concentration is 5 mM);
– optimized buffer.