Description
2X Blitz Mastermix is a ready-to-use mixture for polymerase chain reaction (PCR) with increased accuracy of DNA synthesis. Mastermix contains all the necessary components for PCR, including: thermostable DNA polymerase, dNTP and optimized buffer. The DNA polymerase included in the Blitz mastermix is a chimeric thermostable protein consisting of Pfu polymerase and SSO7d DNA-binding domain. The DNA-binding domain stabilizes the complex of DNA polymerase with the template, it leads to an increase in the processivity, synthesis rate, accuracy and stability of the enzyme in a high ionic strength of the solution. The mastermix has an increased rate of DNA synthesis (up to 3 000 bp/min) and is capable of amplifying DNA fragments longer than 10 000 bp.