Description
Blitz DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The modifications of amino acid structure of the native Pfu results in shorter extension times (20 s/kb), more robust and high yield amplification, and the ability to extend long templates in a fraction of the time, making Blitz a superior choice for cloning. This enzyme is suitable for all PCR applications requiring greater accuracy or long amplicons. The enzyme catalyses the template-dependent polymerization of nucleotides into duplex DNA in the 5’→3’ direction and also exhibits 3’→5’ exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5’ exonuclease activity.