Description
RevM Hot-Start Revertase is a genetically modified reverse transcriptase of Moloney Mouse Leukemia Virus (MMLV), is intended for the synthesis of a complementary DNA strand (cDNA) on a single-stranded RNA template. Due to the presence of several mutations the enzyme possesses inhibited RNase H activity and has increased thermostability. The enzyme possesses reduced activity at temperatures below 37°C and has an optimal working temperature of 55°C. Inactivation occurs after 10 minutes at 80°C. Compared to wild-type MMLV reverse transcriptase RevM provides a higher yield of cDNA, has improved efficiency on GC-rich RNA templates and also has a higher productivity.