The standardization of 3D in vitro model systems in cancer and stem cell research as well as in pharmaceutical applications is an indispensable process due to their potential to resemble the in vivo environment more properly than common 2D systems. Traditional 2D cell cultures offer only restricted cell-cell contacts in one dimension which leads to unnatural cell phenotypes, cell behavior and drug-target interactions. On the other hand, cell spheroids – a dense 3D cell network – allow cell-cell contact in all dimensions for more precise validation of cell based assays. Different technologies are available for development of spheroid-based model systems, which differ in their applications and their potential to standardize 3D based assays for pharmaceutical as well as medical research. In this talk we will compare different commercially available Low-Attachment systems in their ability to produce consistent spheroid data for pharmaceutical and medical research.
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