Description

TaqNovaGC DNA Polymerase is an ideal tool for the amplification of GC-rich templates. A recombinant and  thermostable enzyme isolated from Thermus aquaticus, it is recommended for a wide range of applications requiring DNA synthesis at extremely high temperatures. The TaqNovaGC DNA polymerase is a universal DNA polymerase which works rapidly and effectively in various PCR conditions. The enzyme catalyses DNA synthesis in a 5’-3’ direction, shows no 3’-5’ exonuclease activity, but has a 5’-3’ exonuclease activity.

The application of appropriate PCR buffer conditions and 5x GC-Additive as the PCR enhancer enables the amplification of specific DNA regions with a high GC-content. This PCR additive changes DNA behavior upon heating and can be used with primer-template pairs with a GC-rich content which do not work well under standard PCR conditions. The 5x GC-Additive reduces the number of secondary structures and enables the specific hybridization of primers.

Features and advantages

– Recombinant enzyme of high purity

– Half-life of the enzyme is 45 minutes at 95°C

– Amplifies fragments of up to 5 kb

– Leaves ´A´ overhangs

– High specificity

– Ideal for problematic templates, which fail with standard Taq DNA polymerases

Applications

– Efficient amplification of short and medium size DNA sequences

– GC-rich templates

– Ideal for problematic templates, which fail with standard Taq DNA polymerases