Description
| Name | Part Number | Pack Size |
| RNase H | RT34-025 | 250 U (5 U/µl) |
| RT34-125 | 1250 U (5 U/µl) |
RNase H is a 18.9 kDa recombinant endoribonuclease purified from an Escherichia coli strain, which over-expresses cloned RNase H gene (rnh). The enzyme hydrolyses specifically the phosphodiester bonds of RNA hybridized to DNA and produces 5 ́ phosphate-terminated oligoribonucleotides and single-stranded DNA. RNase H does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as RNA bonded to the cDNA template may prevent binding of the amplification primers in a PCR reaction. RNase H treatment is often necessary when amplifying longer, full-length cDNA targets. In addition, RNase H is useful for the removal of poly(A) tails on mRNAs after hybridization with oligo(dT) and also for the site-specific enzymatic cleavage of RNA.
Applications
– Removal of RNA after first strand cDNA synthesis (RT-PCR and qRT-PCR)
– Removal of mRNA prior to synthesis of second strand cDNA
– Removal of the poly(A) sequences of mRNA after hybridisation with oligo(dT)
– Site-specific cleavage of RNA
– Studies of in vitro polyadenylation reaction products