Description
| Name | Part Number | Pack Size |
| RNase A (DNase Free) | RP145 | 50 mg |
RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates [1,2]. The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA [8]. RNase is supplied as a lyophilized powder.
Properties
– Source: bovine pancreas
– Specific activity: >2500 u/mg protein (>50 Kunitz units/mg protein)
– Molecular Weight: 13.7 kDa monomer
– The working concentration for RNase A is 1-100 µg/ml depending on the application.
Applications
– Plasmid and genomic DNA preparation [3, 4]
– RNase protection assays [3]
– Mapping single-base mutations in DNA or RNA [5, 6]
– Remove unspecifically bound RNA
– Analysis of RNA sequences
– Hydrolyze RNA contained in protein samples
References
1. Blackburn, P., Moore S., Pancreatic ribonuclease, The Enzymes, V, (Boyer, P.D, ed.), Academic Press, New York, the third edition, vol. 15, 317-433, 1982.
2. Raines, R.T., Ribonuclease A, Chem. Rev., 98, 1045-1065, 1998.
3. Sambrook, J., Russell, D.W., Molecular Cloning: A Laboratory Manual, the third edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1.31-1.38, 2001.
4. Sharma, R.C., et al., A rapid procedure for isolation of RNA-free genomic DNA from mammalian cells, BioTechniques, 14, 176-178, 1993.
5. Myers R.M., et al., Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes, Science 230, 1242-1246, 1985.
6. Winter E., et al., A method to detect and characterize point mutations in transcribed genes: Amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells, Proc. Natl. Acad. Sci. USA, 82, 7575-7579, 1985.
7. Kunitz, M.A., A spectrophotometric method for the measurement of ribonuclease activity, J. Biol. Chem., 164, 563-568, 1946.
8. Ausubel, F.M., et al., Current Protocols in Molecular Biology, vol. 1, John Wiley & Sons, Inc., Brooklyn, New York, 3.13.1, 1994-2005.