Phone

01904 900786

Address

The Catalyst, Baird Lane, York, YO10 5GA

M600-1000 ready-to-use DNA Ladder

DNA fragment lengths (bp):

1000, 900, 800, 700, 600

Ready-to-use – premixed with 6x Green DNA Loading Dye for direct loading onto agarose and non-denaturing polyacrylamide gels

Category:

Description

Name Part Number Pack Size
M600-1000 ready-to-use DNA Ladder MR85 100 lanes

Molecular weight standard specially designed for electrophoretic analysis of medium DNA fragments on agarose and non-denaturing polyacrylamide gels. Ideal for sizing of PCR products and medium double-stranded DNA fragments. M600-1000 ready-to-use DNA ladder contain purified blunt-ended PCR products. The ladder is premixed with 6x Green DNA Loading Dye for direct loading onto agarose and non-denaturing polyacrylamide gels.The optimized 6x Green DNA Loading Dye contains two electrophoresis tracking dyes: orange G and xylene cyanol FF. Orange G migrates at approximately 50 bp on a standard 1% TAE or TBE agarose gels, whereas xylene cyanol FF migrates at approx. 4 kbp.

Contents

– M600-1000 ready-to-use DNA Ladder (500 µl)

– 6x Green DNA Loading Dye (500 µl)

Features

– ready-to-use – premixed with 6x Green DNA Loading Dye for direct loading onto agarose and non-denaturing polyacrylamide gels

– allow precise sizing of medium DNA fragments

– easy-to-remember band sizes (differing from each other in the same step size – 100 bp)

– produce sharp bands during gel electrophoresis

– can be visualized under UV light after ethidium bromide, SYBR Green or SYBR Gold staining

– loading dye solution is included

DNA fragment lengths (bp):

1000, 900, 800, 700, 600

Specification

Storage Buffer

10 mM Tris-HCl (pH 7.6), 0.005% xylene cyanol FF, 0.025% orange G, 2.5% Ficoll® 400 and 10 mM EDTA

6x Green DNA Loading Dye (supplied with the ladder)

20 mM Tris-HCl (pH 7.6), 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400 and 60 mM EDTA

Loading

- we recommend loading 10 µl of ready-to-use DNA ladder per gel lane

- do not heat before loading

- mix 1 volume of the dye with 5 volumes of the DNA sample

Quality Control

- tested in appropriate gel electrophoresis

- the absence of nucleases is confirmed by appropriate tests

Storage

Store at room temperature or at 4°C. For longer periods, store at -20°C.

Literature

M600-1000 ready-to-use DNA Ladder