Phone

01904 900786

Address

The Catalyst, Baird Lane, York, YO10 5GA

Ideal DNA Ladder (700-9276 bp)

DNA fragment lengths (bp):

9276, 5982, 3653, 2555, 1827, 1241, 900, 700

Category:

Description

Name Part Number Pack Size
Ideal DNA Ladder (700-9276 bp) MR91 100 lanes

Molecular weight standard specially designed for electrophoretic analysis of medium and large DNA fragments on agarose and non-denaturing polyacrylamide gels. Ideal for sizing of PCR products, digested plasmids and medium or large double-stranded DNA fragments. IDEAL DNA ladder contain purified blunt-ended PCR products and cohesive-ended restriction fragments.

DNA Ladders are supplied with 6x Green DNA Loading Dye (500 µl).

Features

– ideal, because in wide range clear

– allow precise sizing of medium and large DNA fragments

– produce sharp bands during gel electrophoresis

– can be visualized under UV light after ethidium bromide, SYBR Green or SYBR Gold staining

– loading dye solution is included

DNA fragment lengths (bp):

9276, 5982, 3653, 2555, 1827, 1241, 900, 700

Specification

Storage Buffer (TE buffer)

10 mM Tris-HCl (pH 7.6) with 1 mM EDTA

6x Green DNA Loading Dye (supplied with the ladder)

20 mM Tris-HCl (pH 7.6), 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400 and 60 mM EDTA

Loading

- mix 1 volume of the dye with 5 volumes of the DNA solution

- do not heat before loading

- we recommend loading 5 µl of DNA ladder per gel lane

Quality Control

- tested in appropriate gel electrophoresis

- the absence of nucleases is confirmed by appropriate tests

Storage

Store at room temperature or at 4°C. For longer periods, store at -20°C (recommended).

Literature

Ideal DNA Ladder (700-9276 bp) Booklet