|Name||Part Number||Pack Size|
|Gel Loading Dye – BLUE (6X)||AG16||1 mL|
The 6x BLUE DNA Loading Buffer facilitates the loading of DNA onto agarose or polyacrylamide gels. The buffer is concentrated by a factor of six, so in order to obtain the best results mix 2 µl of the loading buffer with 10 µl of the DNA sample. The loading buffer contains Ficoll® 400, which facilitates rapid sinking to the bottom of the well in the agarose or polyacrylamide gel. The solution protects DNA thanks to the presence of EDTA, which inactivates nucleases and other enzymes by binding the divalent metal ions crucial to their activity. The 6x BLUE solution contains bromophenol blue dye which migrate in the electrophoretic field as 300 bp PCR products in a 1% agarose gel.