The EXTRACTME VIRAL RNA KIT utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. During the first isolation step, the material is lysed under highly denaturing conditions to inactivate nucleases and to ensure isolation of intact viral RNA. A homogenate is lysed with guanidine thiocyanate and detergents. RNases are inactivated by guanidine thiocyanate. RNA bounds to a Purification Column membrane by addition of alcohol. A three-step washing stage effectively removes impurities and enzyme inhibitors. Purified RNA is eluted with the use of low ionic strength buffer and may be used directly in all downstream applications, such as RT-PCR, RT-qPCR, cDNA synthesis.