Description

The EXTRACTME DNA SWAB & SEMEN kit is designed for the rapid and efficient purification of high quality genomic DNA from human and animal mucosa membrane swabs (including buccal, nasal, pharyngeal and vaginal swabs) as well as from semen. The isolation protocol and buffer formulations were optimised for high isolation efficiency and DNA purity.

The DNA purification procedure consists of four steps and utilises spin minicolumns with membranes which efficiently and selectively bind nucleic acids. The swab or semen sample is subjected to enzymatic lysis by Proteinase K in the SSL buffer. When isolating from semen, DTT must also be used. In this step, the cell walls, membranes and proteins are degraded by lysis buffer and Proteinase K. After the addition of chaotropic salts, the lysate is applied to the purification column membrane and the DNA is bound. The two-step washing stage effectively removes impurities and enzyme inhibitors. The purified DNA is eluted using either a low ionic strength buffer or water (pH 7.0-9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.

The quality of each production batch (LOT) of the EXTRACTME DNA SWAB & SEMEN kit is tested using standard QC procedures. The purified DNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometer. In addition, the functional quality is tested by qPCR and digestion with restriction enzymes.