Description

Hypernova DNA Polymerase is a unique blend of a modified highly thermostable and proofreading DNA polymerase Pwo isolated from the hyperthermophilic archaeon Pyrococcus woesei and enzymes increasing yield and performance of the PCR reaction. The enzyme mix can generate very long amplicons (over 10 kb). Such long amplicons are often difficult to generate with a single DNA polymerase. Hypernova  is a versatile and easy-to-use polymerase, since it works with many different protocols and requires minimal time consuming optimization. The polymerase produces higher yields than most commercially available enzymes and is ideally suited for difficult PCR templates. For its exceptional stability recommended also for diagnostic PCR in the case when long reactions and high sensitivity are required. The polymerase maintains 95% activity even after 40 cycles consisting of three one minute steps each.

Advantages

– Extreme processivity (for very long amplicons)

– Extreme yield with minimal amounts of enzyme and little optimization

– Extreme fidelity (proofreading activity)

– Ideal for problematic templates that fail with standard Taq DNA polymerases

– Foolproof during multiplex PCR

– Very specific and sensitive

– More thermostable than Taq polymerase

– Very low price!

Applications

– Long range PCR

– Production of higher yields of PCR products

– High fidelity for purposes of cloning, expression, site-directed mutagenesis

– Multiplex PCR

– PCR from GC-rich or difficult templates

– Blunt-end PCR cloning

– Diagnostic PCR