Description

The A+B molecular weight DNA ladder is designed for determining the size of PCR products and large dsDNA fragments in the 500 – 6000 bp range. The A+B DNA Ladder is a mixture of specially selected, purified PCR products. The A+B DNA Ladder contains two ladders, A DNA Ladder and B DNA Ladder, which can be combined in one broad range ladder with a highlighted band (1000 bp). A DNA loading buffer (6x GREEN) is provided with each DNA ladder. The loading buffer contains Ficoll® 400, which facilitates rapid sinking to the bottom of the well in the agarose or polyacrylamide gel. The solution protects DNA thanks to the presence of EDTA, which inactivates nucleases and other enzymes by binding the divalent metal ions crucial to their activity. The 6xGREEN solution contains two different dyes which migrate in the electrophoretic field differently from the standard size PCR products, ensuring that they do not hinder the analysis by image obscuration. The orange G dye corresponds to 50 bp and xylene cyanol FF to 4000 bp PCR products in a 1% agarose gel.

Features and advantages

Allows precise determination of DNA fragments in a wide size range

DNA Ladder A and DNA Ladder B can be used as three different ladders:

A DNA Ladder, or B DNA Ladder for short electrophoresis runs, and A+B DNA Ladder

The combined A+B DNA Ladder contains one band (1000 bp) with the highest intensity, which allows easy identification

Sharp bands are obtained after electrophoresis

Suitable for gels stained with ethidium bromide, SYBR Green or SYBR Gold

Components

A DNA Ladder (250 μl)

B DNA Ladder (250 μl)

6x GREEN DNA Loading Buffer (500 μl)

DNA fragment sizes (bp)

A DNA Ladder: 6000, 3000, 2000, 1000, 500

B DNA Ladder: 3500, 2500, 1500, 1000, 750

A+B Ladder: 6000, 3500, 3000, 2500, 2000, 1500, 1000, 750, 500