Biopixlar® | 3D Single Cell Bioprinting

Biopixlar® | 3D Single Cell Bioprinting

Print tissues with ease

Biopixlar is a completely new type of bioprinter with the unique capability to position cells in three dimensions with high resolution and precision.

Based on innovative Fluicell technology, Biopixlar is capable of generating detailed, multi-cellular biological tissues, directly in native cell culture media.

Biopixlar is all-in-one discovery platform that will help researchers around the globe to build novel tissue models for drug development, disease understanding and regenerative medicine research.

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Description

ALL-IN-ONE DISCOVERY PLATFORM

The Biopixlar platform consists of a bioprinter, a software, a gamepad, all situated on an optical table.

The Biopixlar platform situated on an optical table.

 

EMBEDDED WITH USER-FRIENDLY FEATURES

Biopixlar bioprinter with captions

The platform contains:

1- Bioprinter

The bioprinter includes a micromanipulator arm and a motorized stage that let you precisely position the printhead and the sample. The printhead, based on innovative Fluicell microfluidic technology, is capable of printing several different cell types. The consumable printhead is made from a medical-grade elastomer and includes wells for containing cell suspension, reactant solutions and for collecting waste. The holder is a sophisticated pressure manifold that ensures a closed system, connecting the microfluidic printhead with a precision pressure controller.

The onboard multi-color fluorescence imaging setup allows real-time monitoring of your printing process and post-print analysis. The Biopixlar bioprinter additionally includes built-in environmental air-flow control, printhead heating, illumination of both the full chamber and the surrounding of the printing area, and optional UV light sterilization.

2- Software with gamepad interface

The Biopixlar software enables easy configuration of the bioprinting process and intuitive use of the bioprinter. Through the software, positioning of the printhead can be controlled, as well as cell type, deposition mode, and printing rate. The environmental controls can also be adjusted.

Biopixlar has the capability of bringing complex geometries in an automated or manual manner. The gamepad interface brings an entirely new way to experience bioprinting by putting full control of the process in the palm of your hand. With the gamepad, you have the ability to e.g. position the printhead, and deposit cells with the press of a button. A graphical user interface is also included for design of simple 2D structures.

Single Cell Printing

  • Single-cell resolution

Design your biological tissue by printing down to the level of individual cells.

  • Multi-cellular models

Compose your biological tissue by printing up to three different cell types from the same print-head. By changing the printhead, new cell types can be printed.

  • Real-time monitoring

Follow your printing process in real-time with the fully integrated multi-color fluorescence imaging setup.

  • Ideal for valuable cell sources

Biopixlar is designed for handling scarce and valuable cell sources such as stem cells, primary cells and patient biopsies.

  • High-precision and reproducibility

Structure your biological tissue by precisely controlling the spatial deposition of your living cells and benefit from high reproducibility across replicates.

  • >95 % cell viability

The printing process is optimized to minimize the shear forces on the cell sample, achieving very high cell viability (cell viability was tested across multiple cell lines).

Advance your science with relevant models

 

Biopixlar is designed for:

  • Producing  human-like tissue replicas
  • Understanding of specific diseases
  • Testing of drug compounds
  • Translating new therapies and technologies to patient

Some examples of what you can print

Liver Model

Fluorescence microscopy images of a printed patch of liver cancer cells (HepG2, in red) surrounded by fibroblasts (3T3-J2, in blue) taken at t=0 and 24 hours after printing.

Read more about creating in vitro liver models using Biopixlar.

Skin Cancer Model

Fluorescence microscopy images of four printed patches of skin cancer cells (A431, in pink) surrounded by epithelial cells  (HaCaT, in green) taken at t=0 and 24h after printing.

 

A schematic representation of the Biopixlar printer head printing cells

Place the Biopixlar printhead at a desired spot, and print your cells of interest.  After printing, the cell print is transferred to a cell culture platform allowing for the development of robust tissue models.

 
3D Tissue Model
A fluorescence overlay of the 3D tissue model  printed, with skin cancer cells as a base line (A431, in purple), epithelial cells as a middle layer (HaCaT, in red) and skin cancer cells as a top layer (A431, in green)

Top image: A fluorescence overlay of the 3D tissue model  printed, with skin cancer cells as a base line (A431, in purple), epithelial cells as a middle layer (HaCaT, in red) and skin cancer cells as a top layer (A431, in green). Bottom image: an alternate representation of this layered visualization.

Specification

Bioprinting Performance
Printing Technology Microfluidic hydrodynamic confined flow technology
Printing Dimension 2D and 3D printing
Printing Mode Direct printing of cell suspension without the need of gel matrix
Printing Surface Cell dish with culture medium or buffer
Deposition Mode From individual to thousands cells
Microfluidic Printhead Exchangeable single-use consumable made from a medical grade elastomer. Each has the capacity to hold up to 3 cell wells.
 
Microscope Specifications
Illumination LED fluorescence illumination; bright-field
Fluorescence Filters Blue: Ex. 370-410 nm; Em. 429-462 nm
Green: Ex. 473-491 nm; Em. 502-561 nm
Red: Ex. 580-598 nm; Em. 612-680 nm
Objective Air 10x (Olympus Plan Fluorite Objective, 0.3 NA, 10 mm WD)
Camera 3 Mpx high sensitivity 1920 x 1080
 
Additional Specifications
Stage Travel Range 16 x 16 cm
Dish Holders For 35 mm ø cell dish
For 50 mm ø cell dish
For microtiter plate (6 wells)
Movement Precision 2 micrometers
General Specifications

Schematics drawing of Biopixlar bioprinter with dimensions

Applications

The multicellular in vitro liver model is created by assembling two separate cell layers on top of each other, directly in culture media. The two layers are held together with a connecting cell-adhesive layer, as shown in Figure 1. Figure 2 shows the assembled structure with the arrangement of cells in the two different layers.

Bottom layer The bottom layer consists of a larger uniform patch of 3T3-J2 fibroblasts acting as an underlying support structure.

Top layerThe second layer is the functional part of the model, built from a central patch of hepatocytes (HepG2) with a surrounding fibroblast structure.

Creating detailed bioprinted in vitro models has many important benefits compared to conventional cell culture techniques:

  • Biological relevance – Complex tissue models can be constructed with improved physiological response compared to monoculture systems.
  • Simplicity – Users have direct control of cell placement through Fluicell’s easy-to-use interface.
  • Viability – Printing occurs directly in culture media with minimal mechanical stress on the cells.
liver model assembly

Figure 1. Liver model assembly

 

liver model schematic

Figure 2. Assembled liver model

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