|50X TAE (Tris-acetate-EDTA)
TAE (Tris-acetate-EDTA) is the most commonly used buffer for the agarose gel electrophoresis of nucleic acids. Its highest resolution performance is obtained when separating large DNA fragments (> 2000 bp). If an excision of a gel slice containing a DNA fragment, known as a DNA gel-out, is required, the use of the TAE buffer is recommended.
– As a running buffer for electrophoresis of nucleic acids in agarose gels
– Preparation of agarose gels
– Electrophoresis of large DNA fragments
– DNA gel-out procedures
2.0 M Tris (pH 8.3), 1.0 M acetic acid, 0.05 M EDTA
The TAE buffer has been tested in electrophoretic runs utilizing agarose gels. The absence of nucleases has been confirmed by the relevant QC procedures.
Store at +4°C or room temperature.
Shipping at room temperature
- Prior to use, the stock solution provided should be diluted to the working solution strength.
- In order to obtain a working solution (1x concentrated), dilute one part of the 50x TAE buffer stock solution provided in 49 parts of distilled water; for example, add 980 ml of distilled water for every 20 ml of the stock solution.
- The preparation of a fresh working solution before each electrophoresis run is recommended.