Description
Name |
Part Number |
Pack Size |
10x TG (Tris-Glycine) |
AC18 |
250 mL |
TG (Tris-Glycine) is the buffer most commonly used for the non-denaturating polyacrylamide gel electrophoresis of proteins.
Applications
Non-denaturating polyacrylamide gel electrophoresis of proteins
Specification
Formulation
0.25 M Tris (pH 8.3), 1.92 M glycine
Quality control
The TG buffer has been tested in electrophoretic runs utilizing agarose and polyacrylamide gels. The absence of nucleases and proteases has been confirmed by the relevant QC procedures.
Storage conditions
Store at +4°C or room temperature.
Shipping conditions
Shipping at room temperature
Usage
- Prior to use, the stock solution provided should be diluted to the working solution strength.
- In order to obtain a working solution (1x concentrated), dilute one part of the 10x TG buffer stock solution provided in 9 parts of distilled water; for example, add 450 ml of distilled water for every 50 ml of the stock solution.
- The preparation of a fresh working solution before each electrophoresis run is recommended.
- Non-denaturating polyacrylamide gel electrophoresis of proteins