|10x TG (Tris-Glycine)
TG (Tris-Glycine) is the buffer most commonly used for the non-denaturating polyacrylamide gel electrophoresis of proteins.
Non-denaturating polyacrylamide gel electrophoresis of proteins
0.25 M Tris (pH 8.3), 1.92 M glycine
The TG buffer has been tested in electrophoretic runs utilizing agarose and polyacrylamide gels. The absence of nucleases and proteases has been confirmed by the relevant QC procedures.
Store at +4°C or room temperature.
Shipping at room temperature
- Prior to use, the stock solution provided should be diluted to the working solution strength.
- In order to obtain a working solution (1x concentrated), dilute one part of the 10x TG buffer stock solution provided in 9 parts of distilled water; for example, add 450 ml of distilled water for every 50 ml of the stock solution.
- The preparation of a fresh working solution before each electrophoresis run is recommended.
- Non-denaturating polyacrylamide gel electrophoresis of proteins