Description
| Name | Part Number | Pack Size |
| 10X TBE (Tris-borate-EDTA) | AG19 | 250 mL |
TBE (Tris-borate-EDTA) is a buffer used for the electrophoresis of nucleic acids. Thanks to its higher buffering capacity and lower electric conductivity in comparison to a TAE buffer, the TBE buffer can be used for electrophoresis in both agarose and polyacrylamide gels. It is more suitable for the separation of smaller DNA fragments (< 500 bp) such as PCR products or restriction fragments. If an excision of a gel slice containing a DNA fragment, known as a DNA gel-out, is required, the use of the TAE buffer is recommended. The TBE buffer contains borate, which is a strong inhibitor against such enzymes as DNA ligase or DNA polymerase.
Applications
– As a running buffer for the electrophoresis of nucleic acids in agarose and polyacrylamide gels
– Preparation of agarose and polyacrylamide gels
– High resolution electrophoresis of small DNA fragments