BLIRT ExtractMe DNA Clean-Up 96-well Kit

High-throughput DNA purification after enzymatic reactions in 96-well format

ExtractMe DNA Clean-Up 96-well Kit
Name Part Number Pack Size
ExtractMe DNA Clean-Up 96-well Kit  EM22-192 2x 96 preps
EM22-960 10x 96 preps

 

The EXTRACTME DNA CLEAN-UP 96-WELL kit is designed for the rapid and efficient purification of DNA fragments after enzymatic reactions. It efficiently removes nucleases, enzyme inhibitors, detergents, restriction enzymes, polymerases, divalent ions, salts etc. The purified DNA can be used in common downstream applications. The kit enables the purification of DNA fragments from 50 bp to 30 kb, as well as plasmid and genomic DNA. However purification of fragments smaller than 100 bp and larger than 10 kb will result in decreased recovery rates. The purification protocol and buffer formulations were optimized for high yields and purity of DNA. The product is intended for research use only.

SAMPLE MATERIAL

up to 100 μl of a DNA sample per well

YIELD

85-99%, depending on DNA fragment length (in the range of 100 bp – 10 kb)

BINDING CAPACITY

30 µg DNA per well

DNA FRAGMENT LENGTH

- 100 bp – 10 kb
- DNA fragments in the 50-100 bp and 10-30 kbp range can also be purified, as can genomic and plasmid DNA, however the efficiency will be decreased.

TIME REQUIRED

40 min per plate

DNA PURITY

A260/A280 ratio = 1.7 – 1.9

PLATE FORMAT

standard SBS footprint

EQUIPMENT COMPATIBILITY

- Centrifuge with rotor for plates (≥3k x g);

- Automatic Liquid Handling Systems (eg. Biomek® FX, TECAN Freedom Evo, TECAN Genesis, Eppendorf epMotion)

Principle

The DNA purification procedure utilizes 96-minicolumns plates with membranes which efficiently and selectively bind nucleic acids. In the first step the CB Buffer is added to a DNA sample. It causes proteins to degrade and enables DNA binding to the column membrane. As an added convenience, the binding buffer contains a colour indicator, which facilitates easy monitoring of the solution’s pH for optimal DNA binding. The two-step washing stage efficiently removes impurities and enzyme inhibitors. The purified DNA is eluted using either a low ionic strength buffer (Elution Buffer) or water (pH 7.0-9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth or stored until ready to use.

Quality control

The quality of each production batch (LOT) of the EXTRACTME DNA CLEAN-UP 96-WELL kit is tested using BLIRT’s ISO-certified quality management system. The purified DNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometry. In addition, the functional quality is tested by qPCR.