BLIRT TaqNova-RED Polymerase

TaqNova polymerase with inert red dye, which facilitates accurate low volume pipetting and is an indicator of an enzyme addition

TaqNova-RED Polymerase
Name Part Number Pack Size
TaqNova-RED Polymerase RP100R 1000 U (1 U/µl)
  RP20R 200 U (1 U/µl)

 

TaqNova-RED DNA Polymerase is a 94 kDa recombinant thermostable Taq DNA polymerase,  isolated from Thermus aquaticus. It is recommended for a wide range of applications, which require DNA synthesis in extremely high temperatures. The enzyme is supplemented with one inert gel tracking dye.

TaqNova-RED polymerase is a universal and easy-to-use DNA polymerase, which works rapidly and effectively in various PCR conditions. The enzyme catalyses DNA synthesis in a 5’-3’ directions, it does not show a 3’-5’ exonuclease activity, however it has a 5’-3’ exonuclease activity.

Features:

- recombinant enzyme of high purity

- extreme yield with minimal amounts of enzyme and little optimisation

- catalyses DNA polymerisation at 72°C in the presence of Mg ions

- half-life of the enzyme is 45 minutes at 95°C

- shows 5’-3’ exonuclease activity

- does not have 3’-5’ exonuclease activity

- adds A on the 3’ ends

- visualizes the addition of the DNA polymerase to the PCR reaction

- confirms complete mixing

- enables direct loading of PCR products onto an agarose gel without addition of a gel loading buffer

- the added dye allow to track electrophoresis progress

- do not affect PCR performance

- do not interfere with most downstream applications (exception: the polymerase is not recommended for any downstream applications using absorbance or fluorescence excitation)

Applications:

- efficient amplification of short and medium size sequences

- diagnostic PCR

Concentration:

1 U/µl

Contents:

TaqNova-RED polymerase; 10x TaqNova reaction buffer with KCl; 10x TaqNova reaction buffer with (NH4)2SO4; 50 mM MgCl2

10x TaqNova reaction buffer with KCl (without Mg2+):

100 mM Tris-HCl (pH 8.8, 25°C), 500 mM KCl, 0.8% (v/v) Nonidet P40

10x TaqNova reaction buffer with (NH4)2SO4 (without Mg2+):

750 mM Tris-HCl (pH 8.8 w 25°C), 200 mM (NH4)2SO4, 0,1% (v/v) Tween 20

Storage Buffer:

20 mM Tris-HCl (pH 8.0 at 25°C), 200 mM KCl, 0.1 mM EDTA, 2 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, red dye, 50% (v/v) glycerol.

Storage conditions:

Store at -20ºC.

Quality control:

Nuclease-free. Extensively tested for PCR applications.

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