BLIRT M100-500 DNA Ladder

DNA fragment lengths (bp):

500, 400, 300, 200, 100

M100-500 DNA Ladder
M100-500 DNA Ladder
M100-500 DNA Ladder
M100-500 DNA Ladder M100-500 DNA Ladder M100-500 DNA Ladder
Name Part Number Pack Size
M100-500 DNA Ladder MR71 100 lanes

 

Molecular weight standard specially designed for electrophoretic analysis of small DNA fragments on high percentage agarose (2-3%) and non-denaturing polyacrylamide (8-16%) gels. Ideal for sizing of PCR products and short double-stranded DNA fragments. M100-500 DNA ladder contain purified blunt-ended PCR products.

DNA Ladders are supplied with 6x Green DNA Loading Dye (500 µl).

Features

- allow precise sizing of small DNA fragments

- easy-to-remember band sizes (differing from each other in the same step size - 100 bp)

- produce sharp bands during gel electrophoresis

- can be visualized under UV light after ethidium bromide, SYBR Green or SYBR Gold staining

- loading dye solution is included

DNA fragment lengths (bp):

500, 400, 300, 200, 100

Storage Buffer (TE buffer)

10 mM Tris-HCl (pH 7.6) with 1 mM EDTA

6x Green DNA Loading Dye (supplied with the ladder)

20 mM Tris-HCl (pH 7.6), 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400 and 60 mM EDTA

Loading

- mix 1 volume of the dye with 5 volumes of the DNA solution

- do not heat before loading

- we recommend loading 10 µl of DNA ladder per gel lane

Quality Control

- tested in appropriate gel electrophoresis

- the absence of nucleases is confirmed by appropriate tests

Storage

Store at room temperature or at 4°C. For longer periods, store at -20°C (recommended).

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