BLIRT ExtractMe DNA Clean-Up Kit

Rapid and efficient purification of DNA fragments after enzymatic reactions; the kit enabes the purification of DNA fragments frin 50 bp to 30 bp as well as plasmid and genomic DNA: 90-99% recovery dependng on DNA fragment length (in the range 100bp - 10 kb)

ExtractMe DNA Clean-Up Kit
 Product Part number Pack size
EXTRACTMe DNA Clean-up kit   SWA-EM07-050 50
SWA-EM07-150 150
SWA-EM07-250 250

 

The EXTRACTME  DNA CLEAN-UP kit is designed for the rapid and efficient purification of DNA fragments after enzymatic reactions. It efficiently removes nucleases, enzyme inhibitors, detergents, restriction enzymes, polymerases, divalent ions, salts etc. The purified DNA can be used in common downstream applications. The kit enables the purification of DNA fragments from 50 bp to 30 kb, as well as plasmid and genomic DNA. However purification of fragments  smaller than 100 bp and larger than 10 kb will result in decreased recovery rates.

The DNA purification procedure utilises spin minicolumns with membranes which efficiently and selectively bind nucleic acids. In the first step the CB Buffer is added to a DNA sample. It causes proteins to degrade and enables DNA binding to the column membrane. As an added convenience, the binding buffer contains a colour indicator, which facilitates easy monitoring of the solution’s pH for optimal DNA binding. The two-step washing stage efficiently removes impurities and enzyme inhibitors. The purified DNA is eluted using either a low ionic strength buffer (Elution Buffer) or water (pH 7.0-9.0) and can be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth or stored until ready to use.

The quality of each production batch (LOT) of the EXTRACTME  DNA CLEAN-UP kit is tested using standard QC procedures. The purified DNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometer. In addition, the functional quality is tested by qPCR and digestion with restriction enzymes.

SAMPLE MATERIAL

Up to 100 μl of a DNA sample.

YIELD

90-99%, depending on DNA fragment length (in the range of 100 bp – 10 kb)

DNA FRAGMENT LENGTH

100 bp -  10 kb

DNA fragments in the 50-100 bp and 10-30 kb range can also be purified, as can genomic and plasmid DNA, however the efficiency will be decreased.

BINDING CAPACITY

Approx. 25 μg DNA

TIME REQUIRED

5-10 minutes (including incubation time).           

DNA PURITY

A260/A280 ratio = 1.7 – 1.9